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National Institutes of Health - Eunice Kennedy Shriver National Institute of Child Health and Human Development - Biovisualization
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Rapid Prototyping of Transmission Electron Tomography

Working with Rachid Sougrat and Edwin Fung in the CBMP EM Facility, we created a 3D model of the Golgi Apparatus from Transmission Electron Microscope (TEM) tomography.

electron tomography

Imod was used for aligning the images, ImageJ was used to adjust contrast and brightness in the images, and Amira was used to trace each layer and create the 3D model.

We modified the resulting 3D object and added a base and text, which Darrell Hurt from NIAID printed with a 3D printer. The original model was given to Jennifer Lippincott-Schwartz, who brought it to the Golgi Conference in Pavia, Italy, where the model was inducted into the Camillo Golgi section of the University Science Museum. The model was reprinted a year later, courtesy of Terry Yoo in the Office of High Performance Computing and Communications at the NLM, to showcase at the UCSS Biovisualization Team booth at SIGGRAPH 2009 in New Orleans.

rapid prototype print of golgi apparatus electron tomography

Rapid prototype model of the Golgi Apparatus. The following is printed on the model: Golgi Apparatus, Jennifer Lippincott-Schwartz, Rachid Sougrat, Edwin Fung, Tim Mrozek, Bryan Twomey, John Rouse, Jeremy Swan, Physical Model by Darrell Hurt, Bioinformatics and Computational Biology Branch, OTIS, NIAID.