Credits

December 2018

Editor in Chief

Shana R. Spindler, PhD
shana.spindler@gmail.com

Layout/Design

Nichole Swan
jonasnic@mail.nih.gov

Background Images (from the 2018 NICHD Joint Scientific Retreat)

2: Larissa Erben (Buonanno Lab)

The figure illustrates hybridization to a hippocampal section using a novel sensitive in situ hybridization fluorescent approach that detects, at a single cell level, small differences between alternatively spliced transcripts.

The figure shows the expression of four differentially spliced isoforms of the Neuregulin receptor ErbB4, which is altered in the prefrontal cortex of schizophrenia patients, in hippocampal GABAergic interneurons. The panel is comprised of pseudo-colored hybridization overlays obtained with single probe-pairs targeting unique exon\exon junctions corresponding to either JMa (white), JMb (magenta), CYT-1 (green) or CYT-2 (cyan) ErbB4 isoforms.

3: Daniel Castranova (Weinstein Lab)

Transgenic zebrafish juvenile showing blood vessels in magenta and lymphatic vessels in green.

4: Petros Lab

All the Colors of the Brainbow: 50um vibratome section from Nkx2.1-Cre;Brainbow mouse, in which each MGE-derived cortical interneuron undergoes stoichastic Cre-mediated recombination events resulting in each cell being labeled with a unique hue.

5: Daniel Castranova (Weinstein Lab)

Transgenic zebrafish larva showing blood vessels in red and blood vessel cell nuclei in green.

6: Hyun Min Jung (Weinstein Lab)

The tail of a transgenic zebrafish juvenile showing arteries in red and veins in green.

7: Julian Lui (Baron Lab)

Growth Plate Blossom: Overlapping images of growth plate from the dismal femur of a 2-wks old mouse. Calcein (fluoresces green) was used to label newly formed bone tissue (counterstained with DAPI, blue) as a measurement of bone growth rate. Images were taken from Keyence BZ-X fluorescence microscope. More detail of the study available at Lui et al. Differential aging of growth plate cartilage underlies differences in bone length and thus helps determine skeletal proportions, PLOS Biology 2018.

8: Rosario Vicidomini (Serpe Lab)

Stereo chords – Ventral ganglia from Drosophila larvae

9: Saumitra Dey (Sam) Choudhury (Serpe Lab)

Communication highways – Axons project out from the ventral nerve cord (source), traverse the body wall muscles (as highway cables) and form synapses at specific points (destination). Insets highlight the source, highway cables and destination.

10-11: Daniel Castranova (Weinstein Lab)

The eye of a transgenic zebrafish embryo showing microtubules. (2018 NICHD DIR/DIPHR Scientific Retreat Image Competition Winner)

12: Marina Venero Galanternik (Weinstein Lab)

Dorsal view of a transgenic zebrafish highlighting the meninges.

13: Petros Lab

I Can See Clearly Now: CUBIC clearing performed on 400um sections with Brainbow labeled Nkx+ cells in the cortex.

14: Marina Venero Galanternik (Weinstein Lab)

Dorsal view of a transgenic zebrafish highlighting the meninges.

15: Marina Venero Galanternik (Weinstein Lab)

Dorsal view of the head of a Mexican tetra (Astyanax mexicanus) Larva injected with dextran.

16: Marina Venero Galanternik (Weinstein Lab)

Dorsal view of a transgenic zebrafish highlighting the meninges.

17: Petros Lab

Supersized Sections: Vibratome slice from PVCre;Brainbow mouse that has been expanded about 4x-fold using Protein Retention Expansion Microscopy (proExM), taken with a 10x lens.

18: Hyun Min Jung (Weinstein Lab)

Adult transgenic zebrafish eye showing blood vessels in green and yellow and angiography in magenta.

19: Rosario Vicidomini (Serpe Lab)

Drosophila stereo motor neurons

20: Laura Pillay and Daniel Castranova (Weinstein Lab)

Transgenic zebrafish larvae showing blood vessels in green, blood cells in red, and angiography in magenta.

21: Saumitra Dey (Sam) Choudhury and Rosario Vicidomini (Serpe Lab)

NICHD – fruit flies with red fluorescent eyes indicating a successful genome editing event via CRISPR

22: Rosario Vicidomini (Serpe Lab)

Circuits in the Drosophila cuticle and muscles