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Eunice Kennedy Shriver National Institute of Child Health and Human Development - Program in Cellular Regulation and Metabolism
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Mary Dasso

Mary Dasso's Section on Cell Cycle Regulation uses a variety of metazoan systems, including Xenopus laevis egg extracts, to study mitotic roles of the Ran GTPase, the nuclear pore complex (NPC) and the SUMO family of ubiquitin-like proteins.

These pathways work in an interconnected manner to assure the accurate segregation of chromosomes during mitosis. In the past year, members of the group documented novel roles for both the SUMO pathway and NPC components in the assembly and function of mitotic kinetochores. Particularly the latter aspects of kinetochore function are regulated through Ran.

Publications

Phosphorylation of Xenopus p31(comet) potentiates mitotic checkpoint exit.
Related Articles

Phosphorylation of Xenopus p31(comet) potentiates mitotic checkpoint exit.

Cell Cycle. 2015 Apr 18;:0

Authors: Mo M, Arnaoutov A, Dasso M

Abstract
p31(comet) plays an important role in spindle assembly checkpoint (SAC) silencing. However, how p31(comet)'s activity is regulated remains unclear. Here we show that the timing of M-phase exit in Xenopus egg extracts (XEEs) depends upon SAC activity, even under conditions that are permissive for spindle assembly. p31(comet) antagonizes the SAC, promoting XEE progression into anaphase after spindles are fully formed. We further show that mitotic p31(comet) phosphorylation by Inhibitor of nuclear factor κ-B kinase-β (IKK-β) enhances this role in SAC silencing. Together, our findings implicate IKK-β in the control of anaphase timing in XEE through p31(comet) activation and SAC downregulation.

PMID: 25892037 [PubMed - as supplied by publisher]

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