The assembly of HIV-1 Gag proteins is an essential process for the generation of fully infectious HIV particles. While it is widely accepted that this process is largely mediated by binding of Gag to the viral genome, emerging evidence has shown that Gag can bind to a diverse class of host cell RNAs as well. To date, it is still unclear whether cellular RNAs play a role during HIV-1 assembly. We are currently addressing this question using a myriad of live-cell imaging, biochemistry and fluorescent in situ hybridization (FISH) techniques. By investigating the interactions between the HIV-1 Gag proteins and different classes of RNA, we hope to determine how RNA-protein binding affects HIV-1 assembly and budding.